| Products/Services Used | Details | Operation |
|---|---|---|
| PCR Cloning and Subcloning | … Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198-6805, USA … Guide sequence for XPF (5′- gccggctcgacggattgcca-3′) was cloned into the transfer plasmid, pLentiCRISPR v2 (from …Guide sequence for XPF (5� -gccggctcgacggattgcca-3) wascloned into the transfer plasmid, pLentiCRISPR v2 (from GenScript). | Get A Quote |
Gemcitabine (2'， 2'-difluorodeoxycytidine; dFdC) is a deoxycytidine analog and is used primarily against pancreatic cancer. The cytotoxicity of gemcitabine is due to the inhibition of DNA replication. However， a mechanism of removal of the incorporated dFdC is largely unknown. In this report， we discovered that nucleotide excision repair protein XPF-ERCC1 participates in the repair of gemcitabine-induced DNA damage and inactivation of XPF sensitizes cells to gemcitabine. Further analysis identified that XPF-ERCC1 functions together with apurinic/apyrimidinic endonuclease (APE) in the repair of gemcitabine-induced DNA damage. Our results demonstrate the importance of the evaluation of DNA repair activities... More
