至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

The CysHis zinc finger protein Zfp1 regulates sexual reproduction and virulence in Cryptococcus neoformans.

Fungal Genet. Biol.. 2019; 
FanCheng-Li,HanLian-Tao,JiangSu-Ting,ChangAn-Ni,ZhouZe-Yang,LiuTong
Products/Services Used Details Operation
PCR Cloning and Subcloning … et al. (2005). mCherry was detected by Western blotting using a monoclonal anti-mCherry antibody (GenScript) as described previously (Liu and Xue, 2014). 2.4. Generation of zfp1Δ mutants and their complemented strains … Get A Quote

摘要

Cryptococcus neoformans is a ubiquitous yeast pathogen that often infects the human central nervous system (CNS) to cause meningitis in immunocompromised individuals. Although numerous signaling pathways and factors important for fungal sexual reproduction and virulence have been investigated, their precise mechanism of action remains to be further elucidated. In this study, we identified and characterized a novel zinc finger protein Zfp1 that regulates fungal sexual reproduction and virulence in C. neoformans. qRT-PCR and ZFP1 promoter regulatory activity assays revealed a ubiquitous expression pattern of ZFP1 in all stages during mating. Subcellular localization analysis indicates that Zfp1 is targeted to... More

关键词

Cryptococcus neoformans,Mating,Sexual reproduction,Virulence,Zinc finger pro