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Transgene-Free Genome Editing in Tomato and Potato Plants Using -Mediated Delivery of a CRISPR/Cas9 Cytidine Base Editor.

Int J Mol Sci. 2019; 
VeilletFlorian,PerrotLaura,ChauvinLaura,KermarrecMarie-Paule,Guyon-DebastAnouchka,ChauvinJean-Eric,NoguéFabien,MazierMari
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PCR Cloning and Subcloning … were cloned into the pDicAID_nCas9-PmCDA_NptII_DELLA [5]. First, a 310 bp fragment containing the 3′ end of the AtU6-26 promoter, the 20 bp guide and the sgRNA scaffold were synthesized (Genscript, Piscataway, NJ, USA, for potato; IDT, Skokie, IL, USA, for tomato) … Get A Quote

摘要

Genome editing tools have rapidly been adopted by plant scientists for gene function discovery and crop improvement. The current technical challenge is to efficiently induce precise and predictable targeted point mutations valuable for crop breeding purposes. Cytidine base editors (CBEs) are CRISPR/Cas9 derived tools recently developed to direct a C-to-T base conversion. Stable genomic integration of CRISPR/Cas9 components through -mediated transformation is the most widely used approach in dicotyledonous plants. However, elimination of foreign DNA may be difficult to achieve, especially in vegetatively propagated plants. In this study, we targeted the () gene in tomato and potato by a CBE using -mediate... More

关键词

ALS,Agrobacterium,CRISPR/Cas9,cytidine base editor,potato,tomato,transgene-