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Bacterial Expression System> | The synthesis of full-length genes and oligonucleotide DNA primers, DNA sequencing, and, in few cases, site-directed mutagenesis were carried out by GenScript USA Inc. The genes of the natural red-emitting luciferase from P. hirtus (REPh; railroad worm) and the green-emitting luciferase with blue-shifted emission (GBAv) from A. vivianii were synthesized by GenScript (GenScript USA Inc.). Most of the point mutations were introduced by site-directed mutagenesis , whereas others were ordered from GenScript (all point mutations used to destabilize the octamer). | Get A Quote |
The different colors of light emitted by bioluminescent beetles that use an identical substrate and chemiexcitation reaction sequence to generate light remain a challenging and controversial mechanistic conundrum. The crystal structures of two beetle luciferases with red- and blue-shifted light relative to the green yellow light of the common firefly species provide direct insight into the molecular origin of the bioluminescence color. The structure of a blue-shifted green-emitting luciferase from the firefly Amydetes vivianii is monomeric with a structural fold similar to the previously reported firefly luciferases. The only known naturally red-emitting luciferase from the glow-worm Phrixothrix hirtus exists a... More