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Kinetic characterization of the CH activation step for the lipoxygenase from the pathogenic fungus, M. oryzae: Impact of N-linked glycosylation

Biochemistry. 2019-07; 
Kostenko A, Ray K, Iavarone AT, Offenbacher AR.
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Bacterial Expression System … Materials. The gene encoding MoLOX was optimized for expression in P. pastoris and synthesized by GenScript (Piscataway, NJ) … cerevisiae was gene optimized for recombinant expression in E. coli, synthesized, and subcloned into pET22b vector by GenScript Get A Quote
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摘要

Lipoxygenases from pathogenic fungi belong to the lipoxygenase family of enzymes, which catalyze C-H activation of polyunsaturated fatty acids to form a diverse set of cell-signaling hydroperoxides. While the lipoxygenase catalytic domains are structurally and functionally similar, these fungal enzymes are decorated with N-linked glycans. The impact of N-linked glycans on the structure and function of these enzymes remains largely unknown. One exemplary system is MoLOX, a lipoxygenase from the fungus Magnaporthe oryzae, that is emerging as an important target for the devastating rice blast disease. Herein, we demonstrate that hydrogen transfer, associated with C-H cleavage of the substrate linoleic acid by MoLO... More

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