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Evolutionarily conserved anterior expansion of the central nervous system promoted by a common PcG-Hox program

Development. 2018-04; 
Yaghmaeian Salmani B, Monedero Cobeta I, Rakar J, Bauer S, Curt JR, Starkenberg A, Thor S
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Bacterial Expression System The sequences coding for Hoxb9 and Hoxb13 were codon optimized for chick expression (Genscript). EcoRI site and consensus start codon (Kozak, 1984) were added to the 5′ end. To the 3′ end, stop codons (amb, och, opa) followed by an XbaI site were added (see supplementary Materials and Methods for DNA sequences). DNA was generated by gene synthesis (Genscript), and cloned into plasmid pCAGGS as EcoRI/XbaI fragments. Get A Quote

摘要

A conserved feature of the central nervous system (CNS) is the prominent expansion of anterior regions (brain) compared with posterior (nerve cord). The cellular and regulatory processes driving anterior CNS expansion are not well understood in any bilaterian species. Here, we address this expansion in Drosophila and mouse. We find that, compared with the nerve cord, the brain displays extended progenitor proliferation, more elaborate daughter cell proliferation and more rapid cell cycle speed in both Drosophila and mouse. These features contribute to anterior CNS expansion in both species. With respect to genetic control, enhanced brain proliferation is severely reduced by ectopic Hox gene expression, by eithe... More

关键词

Asymmetric division; Cell cycle; Combinatorial control; Evolution of the CNS; Lineage size; Nervous system development