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Characterization of narrow-leaf lupin (Lupinus angustifolius L.) recombinant major allergen IgE-binding proteins and the natural β-conglutin counterparts in sweet lupin seed species

Food Chem. 2018-04; 
Jimenez-Lopez JC, Foley RC, Brear E, Clarke VC, Lima-Cabello E, Florido JF, Singh KB, Alché JD, Smith PMC
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摘要

β-conglutin has been identified as a major allergen for Lupinus angustifolius seeds. The aim of this study was to evaluate the binding of IgE to five recombinant β-conglutin isoforms (rβ) that we overexpressed and purified and to their natural counterparts in different lupin species and cultivars. Western blotting suggested β-conglutins were the main proteins responsible for the IgE reactivity of the lupin species and cultivars. Newly identified polypeptides from "sweet lupin" may constitute a potential new source of primary or cross-reactive sensitization to lupin, particularly to L. albus and L. angustifolius seed proteins. Several of them exhibited qualitative and quantitative differences in IgE-binding ... More

关键词

Conglutins; Cross-allergenicity; Diagnosis; Food allergy; IgE-binding activity; Immunotherapy; Recombinant allergen; Seed storage proteins; Sweet lupin; Vicilin