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RNA Recognition-like Motifs Activate a Mitogen-Activated Protein Kinase

Biochemistry. 2018-12; 
Phillips T, Tio CW, Omerza G, Rimal A, Lokareddy RK, Cingolani G, Winter E
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Bacterial Expression System ...encoding residues 162–265 of SSP2 that had been codon-optimized for Escherichia coli expression (Genscript) linked to a NotI restriction endonuclease site. Get A Quote

摘要

Smk1 is a mitogen-activated protein kinase (MAPK) family member in the yeast Saccharomyces cerevisiae that controls the postmeiotic program of spore formation. Ssp2 is a meiosis-specific protein that activates Smk1 and triggers the autophosphorylation of its activation loop. A fragment of Ssp2 that is sufficient to activate Smk1 contains two segments that resemble RNA recognition motifs (RRMs). Mutations in either of these motifs eliminated Ssp2's ability to activate Smk1. In contrast, deletions and insertions within the segment linking the RRM-like motifs only partially reduced the activity of Ssp2. Moreover, when the two RRM-like motifs were expressed as separate proteins in bacteria, they activated Smk1. We ... More

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