Products/Services Used | Details | Operation |
---|---|---|
Bacterial Expression System> | Corresponding donor and acceptor plasmids were synthesized by GenScript (Piscataway, New Jersey, USA) or were already part of an existing plasmid library. | Get A Quote |
The generation of monoclonal cell lines is an important early process development step for recombinant protein production. Although single-cell cloning is an established method in mammalian cell lines, straightforward protocols are not yet available for insect cells. We describe a new method for the generation of monoclonal insect cells without using fetal bovine serum and/or feeder cells pretreated by irradiation or exposure to mitomycin. Highly productive clones of Drosophila melanogaster S2 cells were prepared in a two-step procedure, comprising the establishment of a polyclonal population and subsequent single cell isolation by limiting dilution. Necessary growth factors were provided by co-cultivation of s... More