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Bacterial Expression System> | The cDNA encoding for full-length human Hook1 was codon optimized for expression in Sf9 cells and synthesized (Genscript Biotech, Piscataway, NJ)...The cDNAs encoding for human BICD21–98 (UniProt: Q8TD16-1) and Spindly1–142 (UniProt: Q96EA4-1) were synthesized with codon optimization for E. coli expression (Genscript Biotech). | Get A Quote |
Cytoplasmic dynein is the major minus-end-directed microtubule-based motor in cells. Dynein processivity and cargo selectivity depend on cargo-specific effectors that, while generally unrelated, share the ability to interact with dynein and dynactin to form processive dynein-dynactin-effector complexes. How this is achieved is poorly understood. Here, we identify a conserved region of the dynein Light Intermediate Chain 1 (LIC1) that mediates interactions with unrelated dynein-dynactin effectors. Quantitative binding studies map these interactions to a conserved helix within LIC1 and to N-terminal fragments of Hook1, Hook3, BICD2, and Spindly. A structure of the LIC1 helix bound to the N-terminal Hook domain re... More