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Biochemical studies of membrane bound Plasmodium falciparum mitochondrial L-malate: quinone oxidoreductase, a potential drug target

Biochim Biophys Acta Bioenerg. 2018-03; 
Hartuti ED, Inaoka DK, Komatsuya K, Miyazaki Y, Miller RJ, Xinying W, Sadikin M, Prabandari EE, Waluyo D, Kuroda M, Amalia E, Matsuo Y, Nugroho NB, Saimoto H, Pramisandi A, Watanabe YI, Mori M0, Shiomi K0, Balogun EO, Shiba T, Harada S, Nozaki T, Kita K
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Bacterial Expression System The sequence of PfMQO (PF3D7_0616800) was codon optimized for E. coli expression (GenScript) and inserted into pUC57 at the EcoRV restriction site. Get A Quote

摘要

Plasmodium falciparum is an apicomplexan parasite that causes the most severe malaria in humans. Due to a lack of effective vaccines and emerging of drug resistance parasites, development of drugs with novel mechanisms of action and few side effects are imperative. To this end, ideal drug targets are those essential to parasite viability as well as absent in their mammalian hosts. The mitochondrial electron transport chain (ETC) of P. falciparum is one source of such potential targets because enzymes, such as L-malate:quinone oxidoreductase (PfMQO), in this pathway are absent humans. PfMQO catalyzes the oxidation of L-malate to oxaloacetate and the simultaneous reduction of ubiquinone to ubiquinol. It is a memb... More

关键词

Biochemical study; Electron transport chain; Fumarate cycle; Plasmodium; TCA cycle; Ubiquinone