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E. coli HMS174 (DE3) is a sustainable alternative to BL21 (DE3)

Microb Cell Fact. 2018-10; 
Hausjell J, Weissensteiner J, Molitor C, Halbwirth H, Spadiut O
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Bacterial Expression System The gene encoding FHT was codon-optimized for expression in E. coli (Genscript, Piscataway, NJ, USA) and synthesized with the recognition sites for the endonucleases NdeI and XhoI by Invitrogen (Thermo Scientific, Waltham, MA, USA). Get A Quote

摘要

BACKGROUND: Escherichia coli is one of the most widely used hosts for recombinant protein production in academia and industry. Strain BL21(DE3) is frequently employed due to its advantageous feature of lacking proteases which avoids degradation of target protein. Usually it is used in combination with the T7-pET system where induction is performed by one point addition of IPTG. We recently published a few studies regarding lactose induction in BL21(DE3) strains. BL21(DE3) can only take up the glucose-part of the disaccharide when fed with lactose. However, initially additional glucose has to be supplied as otherwise the ATP-related lactose uptake barely happens. Yet, as lactose is an inexpensive compound compar... More

关键词

BL21(DE3); E. coli; HMS174(DE3); IPTG; Lactose; Recombinant protein production