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Tropomyosin Isoform Expression in the Adductor Muscle of the Japanese Pearl Oyster, Pinctada fucata

American Journal of Molecular Biology. 2019-06; 
Daisuke Funabara*, Ayaka Ohta, Jungo Sueyoshi, Satoshi Kanoh
Products/Services Used Details Operation
Bacterial Expression System DNA fragments encoding Pifuc-TM-1 or Pifuc-TM-2, with codon usage optimized for expression in Escherichia coli, were commercially synthesized by GenScript Gene Synthesis Service (GenScript, Piscataway, NJ, USA) Get A Quote

摘要

We determined the full-length primary structure of the tropomyosin (TM)-1 and -2 proteins from the adductor muscle of the Japanese pearl oyster Pinctada fucata (Pifuc-TM-1 and Pifuc-TM-2), and found that they are each composed of 284 amino acid residues. We predicted the gene structure of P. fucata TM (Pifuc-TM) using Splign alignment of our cDNA with genomic sequences and elucidated that Pifuc-TM consists of 10 exons. Exons 1 - 3 and 5 - 10 are used to transcribe Pifuc-TM-1 mRNA, and exons 1 - 4 and 6 - 10 are used to transcribe Pifuc-TM-2 mRNA. Both genes share the same start and stop codons located in exon 1 and exon 10, respectively. Using quantitative real-time PCR, we determined that the Pifuc-TM-1 gene w... More

关键词

Adductor Muscle, Alternative RNA Processing, Catch Contraction, Pearl Oyster, Tropomyosin