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Bacterial Expression System> | amino acids 1-21) was optimized for expression in E. coli (GenScript), and a TEV cleavage site followed by 10 histidine residues were encoded at the C-terminus. This optimized sequence … | Get A Quote |
Uridine insertion deletion editing in kinetoplastid protozoa requires a complex machinery, a primary component of which is the RNA Editing Substrate Binding Complex (RESC). RESC contains two modules termed GRBC (Guide RNA Binding Complex) and REMC (RNA Editing Mediator Complex), although how interactions between these modules and their mRNA and gRNA binding partners are controlled is not well understood. Here, we demonstrate that the ARM/HEAT repeat containing RESC protein, MRB10130, controls REMC association with mRNA- and gRNA-loaded GRBC. High throughput sequencing analyses show that MRB10130 functions in both initiation and 3 to 5 progression of editing through gRNA-defined domains. Editing intermediate... More