Products/Services Used | Details | Operation |
---|---|---|
Bacterial Expression System> | XP_001663083.2) named Aedes Cte f 2 like were produced as recombinant proteins using the BacPowerTM Bacterial Expression System (GenScript, Piscataway, NJ, USA).For cellular experiments, endotoxin was depleted from Cte f 2 with ToxinEraserTM columns according to the manufacturer’s instructions (GenScript)...Removal was confirmed by a quantitative and modified Limulus assay (ToxinSensor Chromogenic LAL; GenScript). | Get A Quote |
BACKGROUND: Papular urticaria (PU) is a common insect bite skin hypersensitivity in tropical countries. In order to gain insight into its causal allergens, we aimed to evaluate cellular and humoral immune responses to the recombinant salivary antigen Cte f 2 from the cat flea Ctenocephalides felis. METHOD: Sixty patients with PU and 27 healthy controls were included in this study. Specific IgE, IgG, IgG1, and IgG4 against Cte f 2 and C. felis extract were determined by ELISA. The T-cell response was analyzed using a carboxyfluorescein succinimidyl ester (CFSE)-based dilution assay and Th1/Th2/Th17 cytokine measurements. In addition, a proteomic analysis of IgG and IgE reactive spots of C. felis extract was per... More