Goose parvovirus (GPV) is the etiological pathogen of Derzsy's disease, causing great economic losses in waterfowl industry. A novel goose parvovirus-related virus (NGPV), which caused short beak and dwarfism syndrome (SBDS), has occurred in China since 2015. In this study, two GPV strains (RC45 and RC70) were isolated from diseased growing period geese (45-day-old and 70-day-old), and one NGPV strain GXN45 was isolated from a 45-day-old Cherry Valley duck in China. To better understand the genetic diversity between GPVs isolated from growing period waterfowls and other classical waterfowl parvoviruses, the complete genomes and main genes were sequenced and analyzed. Full-length genomic sequence alignments demo... More
Goose parvovirus (GPV) is the etiological pathogen of Derzsy's disease, causing great economic losses in waterfowl industry. A novel goose parvovirus-related virus (NGPV), which caused short beak and dwarfism syndrome (SBDS), has occurred in China since 2015. In this study, two GPV strains (RC45 and RC70) were isolated from diseased growing period geese (45-day-old and 70-day-old), and one NGPV strain GXN45 was isolated from a 45-day-old Cherry Valley duck in China. To better understand the genetic diversity between GPVs isolated from growing period waterfowls and other classical waterfowl parvoviruses, the complete genomes and main genes were sequenced and analyzed. Full-length genomic sequence alignments demonstrated that both RC45 and RC70 showed the highest identity with classical GPVs YZ99-6 and SHFX1201, whereas GXN45 shared the highest identity with NGPV SDLC01. Sequence alignment of the inverted terminal repeats (ITRs) region showed that GXN45, RC45 and RC70 had two 14-nucleotide deletions compared to classical GPV virulent B strain and one 14-nucleotide deletion compared to mule duck-origin NGPV M15 strain. Phylogenetic tree analysis of NS and VP1 showed that GXN45 was clustered into a branch with NGPV QH15 strain except for the VP1 amino acid tree; although either RC45 or RC70 formed one separate branch distinct from classical GPV isolates, they were in one big phylogenetic tree branch. This study will contribute to a better understanding of the genetic diversity and molecular characterization of three isolated parvoviruses, and lay the foundation to further study the relationship between mutations of virus genome and viral pathogenicity.
