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Truncated Rep protein of porcine circovirus 2 (PCV2) caused by a naturally occurring mutation reduced virus replication in PK15 cells

BMC Vet Res.. 2019-07; 
Hu Y, Cai X, Zhan Y, Yuan X, Liu T, Tan L, Li Y, Zhang L, Yang L, Liu W, Deng Z, Wang N, Yang Y, Guo S, Wang A
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PCR and Cloning The purified PCR products were cloned into pSP-72 cloning vector (Promega, Madison, WI, USA) and sequenced by genscript (Nanjing, China). Get A Quote

摘要

BACKGROUND: Porcine circovirus 2 (PCV2) is the causative agent of porcine circovirus-associated diseases (PCVADs). The infection of PCV2 is widespread and has serious consequence, thereby causing significant economic losses in the swine industry worldwide. Previously, we found that a strain named YiY-3-2-3 has a naturally occurring point mutation (G710 to A710) in ORF1 region, which leads to a shorten product of the rep gene (945 to 660 base pair). Importantly, the Rep protein is responsible for genome replication of PCV2. To explore the effects of this mutation on the PCV2 replication, in the current study we constructed infectious clone of this IF-YiY-3-2-3, as well as those of its two parental strains of IF-... More

关键词

Infectious clone; PCV2 evolution; Porcine circovirus 2(PCV2); Rescued viruses; Truncated Rep protein; Virus replication