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Expression of Recombinant Human Insulin-like Growth Factor Type 1 (rhIGF-1) in Escherichia coli.

Avicenna J Med Biotechnol. 2015;

IranpoorHamidreza，OmidiniaEskandar，VatankhahVenus，GharanjikVahid，ShahbaziM

Products/Services Used	Details	Operation
PCR Cloning and Subcloning	… Cloning of human insulin-like growth factor type 1 gene in E coli DH5α Cloning vector pUC18 and IGF-1 gene were purchased from GenScript (USA) Initially, optimized IGF-1 gene was sub-cloned into pUC18 Cloning vector …	Get A Quote

摘要

Human insulin-like growth factor type 1 (hIGF-1) is a protein consisting of 70 amino acids (MW=7.6 kDa) and mainly synthesized by liver. Mecasermin (Trade name INCRELEX) is the synthetic form of the protein which is used as an effective treatment for particular disorders such as short stature， type 1 and 2 diabetes， and wound healing. Current study was aimed to investigate the expression of human insulin-like growth factor type1 in Escherichia coli (E. coli) BL21 (DE3) expression system in order to produce an active recombinant form of the protein.