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Generation of Lactococcus lactis capable of coexpressing epidermal growth factor and trefoil factor to enhance in vitro wound healing.

Appl. Microbiol. Biotechnol.. 2015-06; 
HuynhEvanna,LiJu
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Recombinant Antibody Services … Scientific, Inc (Waltham, MA) Protein A agarose resin and Terrific Broth (TB) medium were obtained from Genscript (Piscataway, NJ) and Becton Dickinson Diagnostic Systems (Sparks, MD), respectively Recombinant human … Get A Quote

摘要

Epidermal growth factor (EGF) and trefoil factor 3 (TFF3) are peptides that actively support the restitution and repair of mucosal epithelial barriers. Previous studies have shown that TFF3 enhanced EGF effect in wound healing, suggesting that the combined application of the two factors may be advantageous in clinical tissue repair. Expression of multiple proteins in a single host is a desirable approach in a biotechnological process, allowing to reduce cost and increase production efficiency. The aim of the present study was to study the feasibility of coexpressing EGF and TFF3 in food grade bacteria, Lactococcus lactis (L. lactis). Using an expression construct allowing simultaneous translation of two s... More

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