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Mutational analysis of Trypanosoma brucei RNA editing ligase reveals regions critical for interaction with KREPA2.

PLoS ONE. 2015; 
MehtaVaibhav,SenRajashree,MoshiriHoutan,Salavati
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Mutagenesis Services … A total of 20 individual point mutations were made in TbREL1 by site-directed mutagenesis of TbREL1-pet30a All truncations and point mutations were prepared by GenScript Corporation (Piscataway, NJ) In vitro protein expression … Get A Quote

摘要

The Trypanosoma brucei parasite causes the vector-borne disease African sleeping sickness. Mitochondrial mRNAs of T. brucei undergo posttranscriptional RNA editing to make mature, functional mRNAs. The final step of this process is catalyzed by the essential ligase, T. brucei RNA Editing Ligase 1 (TbREL1) and the closely related T. brucei RNA Editing Ligase 2 (TbREL2). While other ligases such as T7 DNA ligase have both a catalytic and an oligonucleotide/oligosaccharide-binding (OB)-fold domain, T. brucei RNA editing ligases contain only the catalytic domain. The OB-fold domain, which is required for interaction with the substrate RNA, is provided in trans by KREPA2 (for TbREL1) and KREPA1 (for TbREL2... More

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