至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Enthalpy-entropy compensation at play in human copper ion transfer.

Sci Rep. 2015-05; 
NiemiecMoritz S,DingeldeinArtur P G,Wittung-StafshedePern
Products/Services Used Details Operation
Mutagenesis Services … constructed using a PrimeSTAR Mutagenesis Basal Kit (Takara, Shiga, Japan) The DNA fragment containing the gene of ATA-117-Rd11 was synthesized (GenScript USA Inc) The ATA-117-Rd11 gene was amplified with KOD … Get A Quote

摘要

Copper (Cu) is an essential trace element but toxic in free form. After cell uptake, Cu is transferred, via direct protein-protein interactions, from the chaperone Atox1 to the Wilson disease protein (WD) for incorporation into Cu-dependent enzymes. Cu binds to a conserved C XXC motif in the chaperone as well as in each of the cytoplasmic metal-binding domains of WD. Here, we dissect mechanism and thermodynamics of Cu transfer from Atox1 to the fourth metal binding domain of WD. Using chromatography and calorimetry together with single Cys-to-Ala variants, we demonstrate that Cu-dependent protein heterocomplexes require the presence of C but not C . Comparison of thermodynamic parameters for mutant ... More

关键词