Products/Services Used | Details | Operation |
---|---|---|
Mutagenesis Services> | … constructed using a PrimeSTAR Mutagenesis Basal Kit (Takara, Shiga, Japan) The DNA fragment containing the gene of ATA-117-Rd11 was synthesized (GenScript USA Inc) The ATA-117-Rd11 gene was amplified with KOD … | Get A Quote |
Copper (Cu) is an essential trace element but toxic in free form. After cell uptake, Cu is transferred, via direct protein-protein interactions, from the chaperone Atox1 to the Wilson disease protein (WD) for incorporation into Cu-dependent enzymes. Cu binds to a conserved C XXC motif in the chaperone as well as in each of the cytoplasmic metal-binding domains of WD. Here, we dissect mechanism and thermodynamics of Cu transfer from Atox1 to the fourth metal binding domain of WD. Using chromatography and calorimetry together with single Cys-to-Ala variants, we demonstrate that Cu-dependent protein heterocomplexes require the presence of C but not C . Comparison of thermodynamic parameters for mutant ... More