至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Functional importance of two conserved residues in intracellular loop 1 and transmembrane region 2 of Family A GPCRs: insights from ligand binding and signal transduction responses of D1 and D5 dopaminergic receptor mutants.

Cell. Signal.. 2015; 
ZhangBoyang,YangXiaodi,TiberiM
Products/Services Used Details Operation
Mutagenesis Services … The four mutants in which metal-binding site cysteines were changed one by one to alanines in both proteins (ie, Atox1 C1A2 , Atox1 A1C2 , WD4 C1A2 , and WD4 A1C2 ) were created by site-directed mutagenesis (GenScript USA Inc), followed by purification via the same … Get A Quote

摘要

For many G protein-coupled receptors (GPCRs), the role of the first intracellular loop (IL1) and its connections with adjacent transmembrane (TM) regions have not been investigated. Notably, these regions harbor several polar residues such as Ser and Thr. To begin uncovering how these polar residues may contribute to the structural basis for GPCR functionality, we have designed human D1-class receptor mutants (hD1-ST1 and hD5-ST1) whereby all Ser and Thr of IL1 and IL1/TM2 juncture have been replaced by Ala and Val, respectively. Both ST1 mutants exhibited a loss of dopamine affinity but similar binding properties for inverse agonists compared to their parent receptors. As well, these mutations dimini... More

关键词

Dopamine,G protein coupling,G protein-coupled receptor,Intracellular loop 1,Transmembrane region 2,