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Functional studies of the gene slr2049 from Synechocystis sp. PCC6803 and its site-directed mutation.

Gene. 2015-06;

LiuBingjun，ChenSili，Zhan

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DNA Sequencing	… PCR amplification conditions were as follows: initial denaturation at 94°C for 10 min, with 30 cycles of 94°C for 30 s, then 55°C for 30 s and 72°C for 15 min; and a final extension at 72°C for 10 min Sequence determination was completed by GenScript Co (Nanjing, China) …	Get A Quote

摘要

Phycobiliprotein is a homologous family of light-harvesting chromoproteins existing in cyanobacteria， red algae and cryptophytes. Phycobiliprotein is made up of phycobilin and its corresponding apophycobiliprotein， and they are covalently linked by the thioether bond with the bilin lyase. Using the software BLAST， we have found gene slr2049 in Synechocystis sp. PCC6803 homologous to the biliprotein lyase gene cpeS. This paper investigates the protein expressed by gene slr2049 to find the enzymatic activity characteristics. We cloned slr2049 and its related genes cpcB， ho1， and pcyA which are linked with the synthesis of phycocyanin. Special amino acid mutagenesis was performed on slr2049 to construct ... More