Products/Services Used | Details | Operation |
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Monoclonal Antibody Services> | … THE™ GST Antibody (mouse monoclonal conjugated to HRP) was purchased from GenScript (Piscataway, NJ), aliquoted, and stored at −20°C The chromogenic substrate (1 Step TMB‐Blotting Substrate, 3,3′,5,5′‐tetramethylbenzidene) was purchased from … | Get A Quote |
The gaseous plant hormone ethylene regulates many processes of high agronomic relevance throughout the life span of plants. A central element in ethylene signaling is the endoplasmic reticulum (ER)-localized membrane protein ethylene insensitive2 (EIN2). Recent studies indicate that in response to ethylene, the extra-membranous C-terminal end of EIN2 is proteolytically processed and translocated from the ER to the nucleus. Here, we report that the conserved nuclear localization signal (NLS) mediating nuclear import of the EIN2 C-terminus provides an important domain for complex formation with ethylene receptor ethylene response1 (ETR1). EIN2 lacking the NLS domain shows strongly reduced affinity for the rec... More