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An essential role of syntaxin 3 protein for granule exocytosis and secretion of IL-1α, IL-1β, IL-12b, and CCL4 from differentiated HL-60 cells.

J. Leukoc. Biol.. 2015-03; 
NaegelenIsabelle,PlançonSébastien,NicotNathalie,KaomaTony,MullerArnaud,VallarLaurent,TschirhartEric J,BréchardSab
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摘要

Besides their roles in the killing of pathogens, neutrophils have the capacity to package a variety of cytokines into cytoplasmic granules for subsequent release upon inflammatory conditions. Because the rapid secretion of cytokines orchestrates the action of other immune cells at the infection site and thus, can contribute to the development and chronicity of inflammatory diseases, we aimed to determine the intracellular SNARE machinery responsible for the regulation of cytokine secretion and degranulation. From a constructed gene-expression network, we first selected relevant cytokines for functional validation by the CBA approach. We established a cytokine-secretion profile for human neutrophils and ... More

关键词

SNARE proteins,cytokines,extracellular release,gelatinase granules,neutrop