| Products/Services Used | Details | Operation |
|---|---|---|
| DNA Sequencing | … 72 °C for 10 min PCR products were separated on a 2 % agarose gel, and amplicons of interest were cloned into a pGM-T vector (Promega, USA) and sequenced by Genscript Inc, Nanjing, China Removal of poor quality reads … | Get A Quote |
In situ sprouting may result in considerable reduction in quantity and quality of groundnut produce. The most feasible and convenient solution is to develop groundnut cultivars with seed dormancy. No molecular marker or gene associated with this important trait of groundnut has been documented. In the present study, 105 unique cDNA fragments were isolated from two groundnut materials with contrasting dormancy phenotypes using GeneFishing technology. Three of the 16 DEGs were related to seed germination as suggested by BLAST2GO analysis. Differential expression of a gene with functions indicated in seed germination [85-3-1 (oleosin kda-like)] was confirmed by qRT-PCR. We postulate that expression of the gene is ... More
