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A Highly Adaptable Method for Quantification of Peptidyl-tRNA Hydrolase Activity

Analytical & Bioanalytical Techniques. 2015; 
W. Blake Holloway, Hana McFeeters, Adam M Powell, Gnana S Nidadavolu and Robert L McFeeters
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Bacterial Expression System … Bacterial Pth1 enzymes were expressed and purified as previously reported [17-19] Human pth2 was commercially synthesized (GenScript, Piscataway, NJ) and cloned into the pET28b vector (Novagen/EMD Millipore, Billerica, MA) using NcoI and BamHI restriction sites … Get A Quote

摘要

The emerging importance of Peptidyl-tRNA hydrolase (Pth) enzymes necessitates the need for a widely applicable functional assay to further studies of this important enzyme family

关键词

Peptidyl-tRNA hydrolase; Peptidyl-tRNA; Enzyme activity; Quantification; Hydrolysis; Pth1; Pth2
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