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Application of quadruple multiplex PCR detection for beef, duck, mutton and pork in mixed meat

Journal of Food and Nutrition Research. 2015; 
Haining He, Xia Hong, Yusheng Feng, Yongsong Wang, Jin Ying, Qi Liu, Yingwen Qian, Xinkui Zhou, Dongshuai Wang
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Custom DNA/RNA Oligos … basis of ND2 [the Ovis aries (sheep) NADH dehydrogenase subunit 2] and 16S rRNA (duck) gene sequencesThe specific primers were synthesized by GenScript and verified bioinformatically by an Oligo 60 programmer The … Get A Quote

摘要

In this study, we have developed quadruple multiplex PCR assay for meat (beef, duck, mutton and pork) in processed meat products. By mixing four primers in appropriate ratios could be identified by the PCR.

关键词

multiplex PCR; beef; duck; mutton; pork; identification
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