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Corynebacterium glutamicum possesses β-N-acetylglucosaminidase.

BMC Microbiol.. 2016-12; 
MatanoChristian,KolkenbrockStephan,HamerStefanie N,SgobbaElvira,MoerschbacherBruno M,WendischVolk
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Bacterial Expression System A nagZ gene (GenBank Acc. No. CAB11942.1) from B. subtilis 168 (ATCC 23857) was synthesized in a codon optimized version for overexpression in E. coli (Genscript, USA). Get A Quote

摘要

In Gram-positive Corynebacterium glutamicum and other members of the suborder Corynebacterianeae, which includes mycobacteria, cell elongation and peptidoglycan biosynthesis is mainly due to polar growth. C. glutamicum lacks an uptake system for the peptidoglycan constituent N-acetylglucosamine (GlcNAc), but is able to catabolize GlcNAc-6-phosphate. Due to its importance in white biotechnology and in order to ensure more sustainable processes based on non-food renewables and to reduce feedstock costs, C. glutamicum strains have previously been engineered to produce amino acids from GlcNAc. GlcNAc also is a constituent of chitin, but it is unknown if C. glutamicum possesses chitinolytic enzymes.

关键词

Chitinase,Corynebacterium glutamicum,N-acetylglucosaminidase,NagA2,Secre