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Bacterial Expression System> | The gene for XcPrxQ, codon-optimized for expression in E. coli and synthesized by GenScript (Piscataway, NJ), was cloned between the NcoI and HindIII sites of pTHCm(Nelson et al., 2008) to express a non-His-tagged protein. | Get A Quote |
Peroxiredoxins (Prxs) are ubiquitous cysteine-based peroxidases that guard cells against oxidative damage, are virulence factors for pathogens, and are involved in eukaryotic redox regulatory pathways. We have analyzed catalytically active crystals to capture atomic resolution snapshots of a PrxQ subfamily enzyme (from Xanthomonas campestris) proceeding through thiolate, sulfenate, and sulfinate species. These analyses provide structures of unprecedented accuracy for seeding theoretical studies, and reveal conformational intermediates giving insight into the reaction pathway. Based on a highly non-standard geometry seen for the sulfenate intermediate, we infer that the sulfenate formation itself can... More