Fatty acid binding protein 7 (FABP7)， abundant in the embryonic brain， binds with the highest affinity to docosahexaenoic acid (DHA) and is expressed in the early stages of embryogenesis. Here， we have examined the consequences of the exposure to different DHA levels and of the in utero depletion of FABP7 on early rat brain development. Neurodevelopment was evaluated through the contents of two proteins， connexin 43 (Cx43) and cyclin-dependent kinase 5 (CDK5)， both involved in neuroblast proliferation， differentiation， and migration. The dams were fed with diets presenting different DHA contents， from deficiency to supplementation. DHA brain embryos contents already differed at embryonic day 11.... More
Fatty acid binding protein 7 (FABP7)， abundant in the embryonic brain， binds with the highest affinity to docosahexaenoic acid (DHA) and is expressed in the early stages of embryogenesis. Here， we have examined the consequences of the exposure to different DHA levels and of the in utero depletion of FABP7 on early rat brain development. Neurodevelopment was evaluated through the contents of two proteins， connexin 43 (Cx43) and cyclin-dependent kinase 5 (CDK5)， both involved in neuroblast proliferation， differentiation， and migration. The dams were fed with diets presenting different DHA contents， from deficiency to supplementation. DHA brain embryos contents already differed at embryonic day 11.5 and the differences kept increasing with time. Cx43 and CDK5 contents were positively associated with the brain DHA levels. When FABP7 was depleted in vivo by injections of siRNA in the telencephalon， the enhancement of the contents of both proteins was lost in supplemented animals， but FABP7 depletion did not modify phospholipid compositions regardless of the diets. Thus， FABP7 is a necessary mediator of the effect of DHA on these proteins synthesis， but its role in DHA uptake is not critical， although FABP7 is localized in phospholipid-rich areas. Our study shows that high contents of DHA associated with FABP7 are necessary to promote early brain development， which prompted us to recommend DHA supplementation early in pregnancy.
