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Biochemicals> | … 0.3× YPD. Alpha factor was purchased from GenScript and diluted in 0.3× YPD for experiments at concentrations indicated in results. The yeast strain is W303 background with the genotype (Mat A bar1 leu2 uras3 his3 trp1). 37 … | Get A Quote |
We report an in situ biofabrication strategy that conveniently partitions microfluidic networks into physically separated while chemically communicating microchannels with semipermeable biopolymer membranes, which enable the facile generation of static gradients for biomedical applications. The biofabrication of parallel biopolymer membranes was initiated with the dissipation of trapped air bubbles in parallel apertures in polydimethylsiloxane (PDMS) microfluidic devices, followed by tunable membrane growth with precise temporal and spatial control to the desired thickness. Static gradients were generated within minutes and well maintained over time by pure diffusion of molecules through the biofabricated s... More