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A Fluorogenic Screening for Enantio-and Diastereoselectivity of 2-Deoxy-d-ribose-5-phosphate Aldolases

Synlett. 2016;

Pietruszka, J., Bisterfeld, C., Küberl, I., & Dick, M.

Products/Services Used	Details	Operation
PCR Cloning and Subcloning	Genes, coding for DERA (de °C) from P. aerophilum, T. maritima and C. psychrerythraea were ordered as synthetic genes from GenScript, followed by cloning into the pET-21a(+)-vector.	Get A Quote

摘要

A highly sensitive, fluorescence-based selectivity screening system for 2-deoxy-d-ribose-5-phosphate aldolases was realized by installing short, straightforward syntheses to fluorophore-coupled carbohydrates as d-ribose, l-ribose, and d-xylose. The substrates allow the simultaneous determination of enantioselectivity and diastereoselectivity of DERA-catalyzed aldol reactions.