| Products/Services Used | Details | Operation |
|---|---|---|
| Custom DNA/RNA Oligos | … The LAMP primers sequences are shown in Table 1. The oligonucleotide primers were synthesized by GenScript (Nanjing, China). Table 1 Oligonucleotide primers. Primer name. Sequence. Target. Amplicon size (bp). Primer length (bp). P-ND1-F3 … | Get A Quote |
Disclosing the composition of meat products in detail is essential to consumers’ rights. In this study, we developed a rapid method based on loop-mediated isothermal amplification to visibly identify pork DNA in meat products. Four pig specific primers were designed according to the mitochondrial DN1 gene sequence. The analytical sensitivity of the LAMP assay for pork DNA detection is 0.5 pg in our experiment, and the results were not affected by processing temperature. We used the LAMP assay and the industry standard for Chinese entry-exit inspections and quarantines to analyze commercial halal products, and the results were comparable. In conclusion, the LAMP assay has excellent sensitivity and specificity ... More
