Transglutaminases (TGase)， which are synthesized as a zymogen (pro-TGase) in Streptomyces sp.， are important enzymes in the food industry. Because this pro-peptide is essential for the correct folding of Streptomyces TGase， TGase is usually expressed in an inactive pro-TGase form， which is then converted to active TGase by the addition of activating proteases in vitro. In this study， Streptomyces hygroscopicus TGase was actively produced by Streptomyces lividans through promoter engineering and codon optimization.
Transglutaminases (TGase)， which are synthesized as a zymogen (pro-TGase) in Streptomyces sp.， are important enzymes in the food industry. Because this pro-peptide is essential for the correct folding of Streptomyces TGase， TGase is usually expressed in an inactive pro-TGase form， which is then converted to active TGase by the addition of activating proteases in vitro. In this study， Streptomyces hygroscopicus TGase was actively produced by Streptomyces lividans through promoter engineering and codon optimization.
