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Region 4 of Rhizobium etli Primary Sigma Factor (SigA) Confers Transcriptional Laxity in Escherichia coli.

Front Microbiol. 2016; 
SantillánOrlando,Ramírez-RomeroMiguel A,LozanoLuis,ChecaAlberto,EncarnaciónSergio M,DávilaGuill
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Mutagenesis Services After the in silico mutagenesis, the resulting sequences and the pRK415 plasmid DNA were sent to GenScript (NJ, USA). GenScript synthesized, sequenced and cloned mutant sequences into pRK415 (pRKsigAm01, sigAm02, and sigAm03)….The pRK415sigAmutant set was sequenced by GenScript. ...In silico designed sigA mutant sequences and pRK415 plasmid DNA were sent to GenScript (NJ, USA) to be chemically synthesized and cloned into the expression vector. Get A Quote

摘要

Sigma factors are RNA polymerase subunits engaged in promoter recognition and DNA strand separation during transcription initiation in bacteria. Primary sigma factors are responsible for the expression of housekeeping genes and are essential for survival. RpoD, the primary sigma factor of Escherichia coli, a γ-proteobacteria, recognizes consensus promoter sequences highly similar to those of some α-proteobacteria species. Despite this resemblance, RpoD is unable to sustain transcription from most of the α-proteobacterial promoters tested so far. In contrast, we have found that SigA, the primary sigma factor of Rhizobium etli, an α-proteobacteria, is able to transcribe E. coli promoters, al... More

关键词

Rhizobium etli,RpoD,SigA,housekeeping,lax consensus promoter,primary sigma factor,region 4,transcriptional la