Products/Services Used | Details | Operation |
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Mutagenesis Services> | Genes were synthesized by GenScript. gp120s, gp140s, antigen binding fragments (Fabs), and IgGs were expressed in 293 cells and purified...The BG505 SOSIP whole-gene saturation mutagenesis and “rare” amino acid libraries were synthesized by Integrated DNA Technologies and GenScript, respectively. | Get A Quote |
Broadly neutralizing antibodies (bnAbs) against the N332 supersite of the HIV envelope (Env) trimer are the most common bnAbs induced during infection, making them promising leads for vaccine design. Wild-type Env glycoproteins lack detectable affinity for supersite-bnAb germline precursors and are therefore unsuitable immunogens to prime supersite-bnAb responses. We employed mammalian cell surface display to design stabilized Env trimers with affinity for germline-reverted precursors of PGT121-class supersite bnAbs. The trimers maintained native-like antigenicity and structure, activated PGT121 inferred-germline B cells ex vivo when multimerized on liposomes, and primed PGT121-like responses in PGT121 ... More