| Products/Services Used | Details | Operation |
|---|---|---|
| Custom DNA/RNA Oligos | … Shanghai, China). Unless indicated otherwise, siMALAT1‐1 was chosen to knockdown MALAT1 expression. DNA oligonucleotide primers were obtained from GenScript (Nanjing, China). siRNA transfection. siRNA transfection … | Get A Quote |
MALAT1 is a conserved long noncoding RNA whose expression correlates with many human cancers. However， its significance in immunity remains largely unknown. Here， we observe that MALAT1 is upregulated in lipopolysaccharide (LPS)-activated macrophages. Knockdown of MALAT1 increases LPS-induced expression of TNFα and IL-6. Mechanistically， MALAT1 was found to interact with NF-κB in the nucleus， thus inhibiting its DNA binding activity and consequently decreasing the production of inflammatory cytokines. Additionally， abnormal expression of MALAT1 was found to be NF-κB-dependent. These findings suggest that MALAT1 may function as an autonegative feedback regulator of NF-κB to help fine-tune innate im... More
