Co-activator candidate interactions for orphan nuclear receptor NR2E1.

NR2E1 (Tlx) is an orphan nuclear receptor that regulates the maintenance and self-renewal of neural stem cells， and promotes tumourigenesis. Nr2e1-null mice exhibit reduced cortical and limbic structures and pronounced retinal dystrophy. NR2E1 functions mainly as a repressor of gene transcription in association with the co-repressors atrophin-1， LSD1， HDAC and BCL11A. Recent evidence suggests that NR2E1 also acts as an activator of gene transcription. However， co-activator complexes that interact with NR2E1 have not yet been identified. In order to find potential novel co-regulators for NR2E1， we used a microarray assay for real-time analysis of co-regulator-nuclear receptor interaction (MARCoNI) that contains peptides representing interaction motifs from potential co-regulatory proteins， including known co-activator nuclear receptor box sequences (LxxLL motif).