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Complete loss of RNA editing from the plastid genome and most highly expressed mitochondrial genes of Welwitschia mirabilis.

Sci China Life Sci. 2019; 
FanWeishu,GuoWenhu,FunkLexis,MowerJeffrey P,ZhuA
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DNA Sequencing … used. Page 132. 122 RT-PCR products were directly sequenced on both strands at GenScript (NJ, GenScript USA Inc.). Sequences were assembled with CodonCode Aligner version 5.0. 1 (CodonCode Corporation). Newly … Get A Quote

摘要

Comparative genomics among gymnosperms suggested extensive loss of mitochondrial RNA editing sites from Welwitschia mirabilis based on predictive analysis. However, empirical or transcriptome data to confirm this massive loss event are lacking, and the potential mechanisms of RNA site loss are unclear. By comparing genomic sequences with transcriptomic and reverse-transcription PCR sequencing data, we performed a comprehensive analysis of the pattern of RNA editing in the mitochondrial and plastid genomes (mitogenome and plastome, respectively) of W. mirabilis and a second gymnosperm, Ginkgo biloba. For W. mirabilis, we found only 99 editing sites located in 13 protein-coding genes in the mitogenome... More

关键词

RNA editing,Welwitschia,expression levels,massive loss,organelle gen