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Functional characterization of β-adrenergic and insulin receptor heteromers.

Neuropharmacology. 2019; 
SusecMaja,SencanskiMilan,GlisicSanja,VeljkovicNevena,PedersenChristina,DrinovecLuka,StojanJurij,NøhrJane,VreclM
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Mutagenesis Services … (Montreal, Canada) and cloned into the vector pcDNA3.1 (+) using the NheI/XbaI restriction sites. GFP 2 -βarr2 1–185 (E186-stop; GAA-TAA) and IR 1-1271-RLuc8 were generated by site-directed mutagenesis and verified by sequencing at Genscript USA Inc … Get A Quote

摘要

This study aimed to functionally characterize β-adrenergic (βAR) and insulin receptor (IR) heteromers in regard to β-arrestin 2 (βarr2) recruitment and cAMP signaling and to examine the involvement of the cytoplasmic portion of the IR β chain in heteromerization with βAR. Evidence for βAR:IR:βarr2 complex formation and the specificity of the IR:βarr2 interaction was first provided by bioinfomatics analysis. Receptor-heteromer investigation technology (HIT) then provided functional evidence of βAR:IR heterodimerization by showing isoproterenol-induced but not insulin-induced GFP-βarr2 recruitment to the βAR:IR complex; the IR:βarr2 interaction was found to only be constitutive. The constitutive IR:... More

关键词

3D visualization,BRET,Heteromer identification technology,Informational spectrum method,Insulin receptor,β -adrenergic rece