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Nesfatin-1 regulates glucoregulatory genes in rainbow trout (Oncorhynchus mykiss).

Comp. Biochem. Physiol., Part A Mol. Integr. Physiol.. 2019; 
BertucciJuan Ignacio,BlancoAyelén Melisa,UnniappanS
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Biochemicals … 2.4. Reagents. Goldfish synthetic nesfatin-1 (VPISIDKTKVKLPEETVKESPQNVDT GLHYDRYLREVIDFLEKDQHFREKLHNTDMEDIKQGKLAKELDFVSHHVRTKLDEL; GenScript, Piscataway, USA) was reconstituted in ultrapure water … Get A Quote

摘要

The aim of this work was to determine if the anorexigen nesfatin-1 modulates the expression of genes involved in glucoregulation in rainbow trout. First, the nesfatin-1 sequence from trout was confirmed. Second, the effects of 0.1, 1 and 10 nM nesfatin-1 on insulin, glucagon, igf-I, igf-II, glut1, glut2, glut4 and sglt1 expression were tested in cultured liver, gut, muscle and adipose tissue. In liver, the expression of insulin and glucagon isoforms X1 increased after 2 h of incubation with 0.1 nM nesfatin-1, while insulin and glucagon X2 expression increased after 4 h with 1 nM treatment. All nesfatin-1 doses tested decreased glut2 expression after 4 h. In adipose tissue... More

关键词

Glucagon,Glucose metabolism,Glucose uptake,Insulin,Insulin-like growth factor,Nesfat