To study the effect of miRNA-200b on hepatic fibrosis induced by CCl in mice. The C59BL/6 mice were randomly divided into three groups (normal control [NC]， CCLR model [Model]， and CCl + miRNA-200b [miRNA]). The hepatic fibrosis was induced by CCl injected subcutaneously twice per week in Model and miRNA groups. After 6 weeks building model， the mice of miRNA group were injected the miRNA-200b from caudal vein twice per week. The mice of Model and miRNA groups were continuously fed for 3 weeks. The IL-1β， IL-6， and TNF-α concentrations of serum were measured by enzyme-linked immunosorbent assay. The hepatic tissues of difference groups were observed by hematoxylin and eosin (H&E) staining， ... More
To study the effect of miRNA-200b on hepatic fibrosis induced by CCl in mice. The C59BL/6 mice were randomly divided into three groups (normal control [NC]， CCLR model [Model]， and CCl + miRNA-200b [miRNA]). The hepatic fibrosis was induced by CCl injected subcutaneously twice per week in Model and miRNA groups. After 6 weeks building model， the mice of miRNA group were injected the miRNA-200b from caudal vein twice per week. The mice of Model and miRNA groups were continuously fed for 3 weeks. The IL-1β， IL-6， and TNF-α concentrations of serum were measured by enzyme-linked immunosorbent assay. The hepatic tissues of difference groups were observed by hematoxylin and eosin (H&E) staining， sirius red staining， Masson staining， and terminal deoxynucleotidyl transferase dUTP nick end labeling assay and measured toll-like receptor 4 (TLR4) and nuclear factor-κB (NF-κB) proteins expressions by western blot assay. The correlation between miRNA-200b and TLR4 were analyzed by dual luciferase target assay. Compared with NC group， the interleukin-1β (IL-1β)， IL-6， and tumor necrosis factor-α (TNF-α) concentrations of Model group were significantly upregulated (P < 0.05， respectively). With miRNA-200b overexpression， the IL-1β， IL-6， and TNF-α concentrations were significantly suppressed (P < 0.05， respectively). The pathologies were improved by H&E staining， sirius red staining， and Masson staining; meanwhile， the hepatic cell apoptosis rate was significantly suppressed (P < 0.05). The TLR4 and NF-κB protein expressions of miRNA group were significantly suppressed compared with the Model group (P < 0.05， respectively). By dual luciferase target assay， the TLR4 was a target gene of miRNA-200b. The miRNA-200b upregulation improved hepatic fibrosis induced by CCl via regulation of TLR4 in vivo.
