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Custom DNA/RNA Oligos> | … Article This article is protected by copyright. All rights reserved. EMSA and western blotting Probe oligonucleotides were synthesised by Genscript Biotechnology Corporation (Nanjing, China). DNA labelling was performed using a Pierce Biotin 3' End DNA Labelling Kit … | Get A Quote |
Xanthomonas oryzae pv. oryzae (Xoo) causes a damaging bacterial leaf blight disease in rice. Cold shock proteins (Csps) are highly conserved nucleic acid-binding proteins present in various bacterial genera, but relatively little is known about their functions in Xanthomonas. Herein, we identified four Csps (CspA-CspD) in the Xoo PXO99 strain. Deletion of cspA decreased cold adaptation and a few known pathogenic factors, including bacterial pathogenicity, biofilm formation and polysaccharide production. Furthermore, we performed transcriptomic and chromosome immunoprecipitation (ChIP) experiments to identify direct targets of CspA and to determine its DNA-binding sequence. Integrative data analysis re... More