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Mechanistic Studies of the Kinase Domains of Class IV Lanthipeptide Synthetases.

ACS Chem. Biol.. 2019; 
HegemannJulian D,ShiLiuqing,GrossMichael L,van der DonkWilfr
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Custom DNA/RNA Oligos … Biolabs. Oligonucleotide primers were purchased from Integrated DNA Technologies. Synthetic peptides used in this study were ordered with >95% purity from Genscript. D2O was purchased from Cambridge Isotopic Laboratories … Get A Quote

摘要

Lanthipeptides, which belong to the superfamily of ribosomally synthesized and posttranslationally modified peptides (RiPPs), are associated with various interesting biological activities. Lanthipeptides can be subdivided into four classes that are defined by the characteristics of the corresponding posttranslational modification enzymes. Class IV lanthipeptide synthetases consist of an N-terminal lyase, a central kinase, and a C-terminal cyclase domain. Here, we present the first in-depth characterization of such a kinase domain from the globisporin maturation enzyme SgbL that originates from sp. NRRL B-2293. Catalytic residues were identified by alignments with homologues and structural modeling. T... More

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