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Bacterial Expression System> | … hyopneumoniae cultures. Protein Expression and Purification. Mhp Eno (MHP168_271) was synthesized by GenScript Biotech Corp. (Nanjing) and expressed using the pET21a vector in the BL21(DE3) E. coli strain. The bacterial … | Get A Quote |
Enolase is an evolutionarily conserved enzyme involved in the processes of glycolysis and gluconeogenesis. belongs to , whose species are wall-less and among the smallest self-replicating bacteria, and is an important colonizing respiratory pathogen in the pig industry worldwide. enolase ( Eno) expression is significantly increased after infection and was previously found to be a virulence factor candidate. Our studies show that Eno is a cell surface-localized protein that can adhere to swine tracheal epithelial cells (STECs). Adhesion to STECs can be specifically inhibited by an Eno antibody. Eno can recognize and interact with plasminogen with high affinity. Here, the first crystal structure of the... More