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Bacterial Expression System> | … NaRs containing specific targeting sequences were codon-optimized and synthesized by GenScript (Piscataway Township, NJ), using human-optimized codons for oocytes and ND7/23 cells and E. coli-optimized codons for pH assays and protein purification … | Get A Quote |
Light-driven sodium pumps (NaRs) are microbial rhodopsins that utilize light energy to actively transport sodium ions out of the cell. Here, we used targeted mutagenesis and electrophysiological methods in living cells to demonstrate that NaRs can be converted into light-activated cation channels by molecular engineering. Specifically, introduction of the R109Q mutation into the sodium ion pump of Dokdonia eikasta (KR2) results in passive ion conductance, with a high preference for potassium over sodium ions. However, in this mutant, residual active outward pumping of sodium ions competes with passive inward transport of potassium. Channel-like behavior could also be achieved by introduction of other ... More