至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Engineered Passive Potassium Conductance in the KR2 Sodium Pump.

Biophys. J.. 2019; 
VogtArend,SilapetereArita,GrimmChristiane,HeiserFlorian,Ancina MöllerMaximiliano,HegemannP
Products/Services Used Details Operation
Bacterial Expression System … NaRs containing specific targeting sequences were codon-optimized and synthesized by GenScript (Piscataway Township, NJ), using human-optimized codons for oocytes and ND7/23 cells and E. coli-optimized codons for pH assays and protein purification … Get A Quote

摘要

Light-driven sodium pumps (NaRs) are microbial rhodopsins that utilize light energy to actively transport sodium ions out of the cell. Here, we used targeted mutagenesis and electrophysiological methods in living cells to demonstrate that NaRs can be converted into light-activated cation channels by molecular engineering. Specifically, introduction of the R109Q mutation into the sodium ion pump of Dokdonia eikasta (KR2) results in passive ion conductance, with a high preference for potassium over sodium ions. However, in this mutant, residual active outward pumping of sodium ions competes with passive inward transport of potassium. Channel-like behavior could also be achieved by introduction of other ... More

关键词