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Bacterial Expression System> | … restriction sites, and sequencing of the final construct was performed by a commercial vendor (GenScript, USA). We transformed the expression construct into E. coli BL21(DE3) cells (New England Biolabs, USA) using standard heat shock … | Get A Quote |
The faster a disease can be diagnosed, the sooner effective treatment can be initiated, motivating a drive to replace standard laboratory techniques with point-of-care technologies that return answers in minutes rather than hours. Thus motivated, we describe the development of an E-DNA scaffold sensor for the rapid and convenient measurement of antibodies diagnostic of syphilis. To achieve this (and in contrast to previous sensors of this class, which relied on single, linear epitopes for detection), we utilized a near full-length antigen as the sensor's recognition element, allowing us to simultaneously display multiple epitopes. The resultant sensor is able to detect antibodies against Treponema... More