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High-Throughput Quantification of Adeno-Associated Virus Transduction with Barcoded Non-Coding RNAs.

Hum. Gene Ther.. 2019; 
XuMeiyu,LiJia,XieJun,HeRan,SuQin,GaoGuangping,TaiPhillip
Products/Services Used Details Operation
PCR Cloning and Subcloning … Reverse: 5'‐CCAGGGTAATGGTCACCAAGTGTTTTTCCCAGGTCA‐3' (BstXI, underlined). The 0.5‐kb bcTuD scaffold cassette was generated via synthesis and propagated in a standard pUC57 cloning vector by GenScript (Piscataway, NJ). The scaffold contains two … Get A Quote

摘要

Recombinant adeno-associated viruses (rAAVs) have become favorable gene delivery vehicles for expressing therapeutic transgenes. Capsid engineering efforts to produce novel AAVs with improved transduction efficiencies, unique tissue specificities, and reduced host immunities are a direct response to the high demand for treatment needs that preexisting rAAVs cannot currently fulfill. New AAV capsids discovered by directed evolution methods, design, or from natural proviral sequences ultimately require extensive characterization in relevant models. Consequently, quantitative screening of candidate capsid libraries now requires reliable high-throughput sequencing approaches. In this study, we have de... More

关键词

adeno-associated virus (AAV),barcoded ncRNA,capsid library screens,tough decoy (