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Integrating multiple biomarkers to increase sensitivity for the detection of Onchocerca volvulus infection.

J. Infect. Dis.. 2019; 
BennuruSasisekhar,Oduro-BoatengGeorgiette,OsigweChinweoke,Del-VallePriscilla,GoldenAllison,OgawaGuilherme Maerschner,CamaVitaliano,LustigmanSara,NutmanThom
Products/Services Used Details Operation
PCR Cloning and Subcloning … Selected proteins (OVOC10469 (A0A044QSR3) and OVOC3261 (A0A2K6VY22)) were expressed recombinantly as C-terminal His-tagged proteins (Genscript, Piscataway NJ) by cloning the genes encoding the proteins into pET30a expression vectors … Get A Quote

摘要

Serological assessments for human onchocerciasis are based on IgG4 reactivity against the OV-16 antigen, with sensitivity around 60-80%. We previously identified 7 novel proteins that could enhance onchocerciasis sero-diagnosis. Further screening by luciferase immunoprecipitation assays identified OVOC10469 and OVOC3261 as the most promising candidates. IgG4-based ELISA using recombinant proteins, yielded sensitivities of 53% for rOVOC10469 and 78% for rOVOC3261, while specificity for each was >99%. Moreover, the newly-identified biomarkers detected some (but not all) of the mf-positive samples not detected by OV-16. The new antigens in combination with OV-16, increased the sensitivity for patent infe... More

关键词

OV-16,OVOC3261,biomarkers,diagnostics,onchocerci